- Conan Edogawa/Jimmy Kudo (江戶川柯南/工藤新一)
After a drug transformed him from teen-aged Jimmy Kudo into an 8 year-old boy, young Conan was still determined to continue fighting crime while trying to get back to his true form. He uses his watch, which shoots out poisonous darts and his bow tie voice changer to aid him on his journey.
- Ami Yoshida (吉田步美)
Is part of the Junior Detective League that was created by young Conan. She is very lively and brave.
- Rachel Moore (毛利蘭)
is Jimmy Kudo’s girlfriend. She’s captain of her high school Karate team, and has a temper rivaled by few. When Dr. Agasa leaves little Conan in her care, she is unaware that he’s really Jimmy shrunken down.
- Dr. Agasa (阿笠博士)
As the only person who knows that Conan is really Jimmy, he comes to the rescue whenever others get close to finding out the truth. He is always creating something that he thinks will help Conan, from glasses with a built in map to a voice changing bow-tie!
- Detective Richard Moore (偵探 毛利小五郎)
is a former policeman and a single father. He’s not the brightest detective and most of his cases are solved by Conan without him knowing about it.